c 1000 touch thermal cycler (Bio-Rad)
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c 1000 touch thermal cycler
C 1000 Touch Thermal Cycler, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 97/100, based on 4832 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/thermal+cycler+c+1000+touch+system/C1000+Touch+Thermal+Cycler+with+96%E2%80%93Deep+Well+Reaction+Module/pm41595780-98-12-16
Average 97 stars, based on 4832 article reviews
C 1000 Touch Thermal Cycler, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 97/100, based on 4832 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/thermal+cycler+c+1000+touch+system/C1000+Touch+Thermal+Cycler+with+96%E2%80%93Deep+Well+Reaction+Module/pm41595780-98-12-16
Average 97 stars, based on 4832 article reviews
c 1000 touch thermal cycler - by Bioz Stars,
2026-09
97/100 stars
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Real-time Polymerase Chain Reaction:Article Title: Acacetin Prevents Bone Loss by Disrupting Osteoclast Formation and Promoting Type H Vessel Formation in Ovariectomy-Induced Osteoporosis Article Snippet: .. Real-time PCR assays for mRNA analysis were performed using a SYBR Premix Ex TaqII kit with a Article Title: Long noncoding RNA Lnc-DIF inhibits bone formation by sequestering miR-489-3p Article Snippet: 1μg of total RNA was used for cDNA synthesis using one step PrimeScript RT reagent kit (TaKaRa, RR037A, Dalian, China). .. Quantitative PCR amplification was performed using the Article Title: Angelicae dahuricae radix alleviates simulated microgravity induced bone loss by promoting osteoblast differentiation Article Snippet: The RNA samples were then converted into cDNA through reverse transcription using the PrimeScript RT reagent kit (TaKaRa, Japan). .. Subsequently, gene expression analysis was performed using real-time PCR with the SYBR Premix Ex TaqII kit (TaKaRa, Japan) on the Article Title: Microgravity Inhibits Cell Proliferation and Promotes Senescence and Apoptosis in Embryonic Stem Cells Article Snippet: Reverse transcription, using RNA as the template, was performed to synthesize cDNA, employing the Evo M-MLV RT Premix for qPCR (Accurate Biotechnology (Hunan) Co., Ltd., Changsha, China). .. Gene expression was assessed by performing real-time PCR analysis with the 2x SYBR Green qPCR master mix (Bimake, Houston, USA) and the Article Title: Bergamottin promotes osteoblast differentiation and bone formation via activating the Wnt/β-catenin signaling pathway. Article Snippet: Osteoporosis is one of the most common bone disorders that seriously affect the health and life quality of elderly individuals.. Reduced osteoblast differentiation and bone formation lead to changes in bone volume and microarchitecture, leaving the bones vulnerable to fracture.. Bergamottin (BM) is a natural compound derived from various citrus fruits and possesses multiple biological activities including antiadipogenesis function. Article Title: MiR-12200-5p Targets Multiple Members of Wnt Signaling Pathway to Inhibit Osteoblast Differentiation and Bone Formation. Article Snippet: Background: Osteoporosis is widespread and has become an emerging problem in the elderly.. MicroRNAs could affect osteoblast differentiation and further regulate the occurrence of osteoporosis by targeting osteogenic differentiation signaling pathways.. Our screening study found that miR-12200-5p simultaneously targeted six important factors within the Wnt signaling pathway (Apc, Tcf4, Tcf7, Wnt3a, Wnt5a, and Lrp6), indicating that miR-12200-5p might function as a strong regulator of this pathway. Reverse Transcription:Article Title: miR-202-5p mitigates the advancement of osteoporosis through the regulation of PTEN Article Snippet: Total RNA was isolated using Trizol (Invitrogen, USA). .. Reverse transcription and quantification of miRNAs were conducted using the TaqMan microRNA Detection Kit (Applied Biosystems, USA). qRT-PCR reactions were carried out on the Article Title: miR-202-5p mitigates the advancement of osteoporosis through the regulation of PTEN. Article Snippet: Human fetal osteoblasts (hFOBs) were used as the research object in this study. hFOBs (Chinese Academy of Medical Sciences, China) were cultured according to the provided instructions. miR-NC, miR-202-5p inhibitor, si-NC, and si-PTEN constructs were purchased from GenePharma (Shanghai, China). miRNA and siRNA constructs were transfected into cells. qRT-PCR Total RNA was isolated using Trizol (Invitrogen, USA). .. Reverse transcription and quantification of miRNAs were conducted using the TaqMan microRNA Detection Kit (Applied Biosystems, USA). qRT-PCR reactions were carried out on the Quantitative RT-PCR:Article Title: miR-202-5p mitigates the advancement of osteoporosis through the regulation of PTEN Article Snippet: Total RNA was isolated using Trizol (Invitrogen, USA). .. Reverse transcription and quantification of miRNAs were conducted using the TaqMan microRNA Detection Kit (Applied Biosystems, USA). qRT-PCR reactions were carried out on the Article Title: miR-202-5p mitigates the advancement of osteoporosis through the regulation of PTEN. Article Snippet: Human fetal osteoblasts (hFOBs) were used as the research object in this study. hFOBs (Chinese Academy of Medical Sciences, China) were cultured according to the provided instructions. miR-NC, miR-202-5p inhibitor, si-NC, and si-PTEN constructs were purchased from GenePharma (Shanghai, China). miRNA and siRNA constructs were transfected into cells. qRT-PCR Total RNA was isolated using Trizol (Invitrogen, USA). .. Reverse transcription and quantification of miRNAs were conducted using the TaqMan microRNA Detection Kit (Applied Biosystems, USA). qRT-PCR reactions were carried out on the Amplification:Article Title: Long noncoding RNA Lnc-DIF inhibits bone formation by sequestering miR-489-3p Article Snippet: 1μg of total RNA was used for cDNA synthesis using one step PrimeScript RT reagent kit (TaKaRa, RR037A, Dalian, China). .. Quantitative PCR amplification was performed using the Article Title: MiR-12200-5p Targets Multiple Members of Wnt Signaling Pathway to Inhibit Osteoblast Differentiation and Bone Formation. Article Snippet: Background: Osteoporosis is widespread and has become an emerging problem in the elderly.. MicroRNAs could affect osteoblast differentiation and further regulate the occurrence of osteoporosis by targeting osteogenic differentiation signaling pathways.. Our screening study found that miR-12200-5p simultaneously targeted six important factors within the Wnt signaling pathway (Apc, Tcf4, Tcf7, Wnt3a, Wnt5a, and Lrp6), indicating that miR-12200-5p might function as a strong regulator of this pathway. Gene Expression:Article Title: Angelicae dahuricae radix alleviates simulated microgravity induced bone loss by promoting osteoblast differentiation Article Snippet: The RNA samples were then converted into cDNA through reverse transcription using the PrimeScript RT reagent kit (TaKaRa, Japan). .. Subsequently, gene expression analysis was performed using real-time PCR with the SYBR Premix Ex TaqII kit (TaKaRa, Japan) on the Article Title: Microgravity Inhibits Cell Proliferation and Promotes Senescence and Apoptosis in Embryonic Stem Cells Article Snippet: Reverse transcription, using RNA as the template, was performed to synthesize cDNA, employing the Evo M-MLV RT Premix for qPCR (Accurate Biotechnology (Hunan) Co., Ltd., Changsha, China). .. Gene expression was assessed by performing real-time PCR analysis with the 2x SYBR Green qPCR master mix (Bimake, Houston, USA) and the SYBR Green Assay:Article Title: Microgravity Inhibits Cell Proliferation and Promotes Senescence and Apoptosis in Embryonic Stem Cells Article Snippet: Reverse transcription, using RNA as the template, was performed to synthesize cDNA, employing the Evo M-MLV RT Premix for qPCR (Accurate Biotechnology (Hunan) Co., Ltd., Changsha, China). .. Gene expression was assessed by performing real-time PCR analysis with the 2x SYBR Green qPCR master mix (Bimake, Houston, USA) and the |